Abstract
Abstract Subunit I of the RNA phage-specific Qβ replicase is shown to be identical with the Escherichia coli 30 S ribosomal protein Sl by the following criteria: (a) ability to restore Qβ RNA-directed activity of Qβ replicase lacking subunit I, (b) immunological cross-reactivity, (c) identity of the first four amino acids at the NH2 terminus, and (d) inhibition of R17 RNA- and poly(U)-dependent amino acid incorporation. The mobility of both proteins in sodium dodecyl sulfate polyacrylamide gel electrophoresis as well as their amino acid composition is indistinguishable.
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