Abstract
Introduction Evaluation of particular lymphocyte subsets in peripheral blood plays a significant role in diagnostics of many pediatric disorders. The knowledge of relative and absolute values of particular lymphocyte lineages in healthy population is essential to analyze results and to evaluate observed changes in immune system. Aim The current study aimed at thorough evaluation of relative and absolute values of lymphoid cells (T and NK cell subsets) in peripheral blood of healthy children. Material and methods The values of different lymphocyte subsets were evaluated using 6-color flow cytometry with regard to age and gender in children aged 3–19 years. Results The results presented in the manuscript might serve as reference to assess the results of patients with immunological abnormalities in the course of various disorders. To our knowledge, this is the first Polish publication evaluating in such detail the composition of various subsets of mononuclear cells in peripheral blood in children.
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