Sublethal Effects of Dermal Exposure to Poly- and Perfluoroalkyl Substances on Postmetamorphic Amphibians.

Abstract

Studies of the toxicity of poly- and perfluoroalkyl substances (PFAS) on amphibians, especially after metamorphosis, are limited. We examined effects of dermal PFAS exposure (30 d) on survival and growth of juvenile American toads (Anaxyrus americanus), eastern tiger salamanders (Ambystoma tigrinum), and northern leopard frogs (Rana pipiens). Chemicals included perfluorooctanoic acid, perfluorooctane sulfonate (PFOS), perfluorohexane sulfonate (PFHxS), and 6:2 fluorotelomer sulfonate (6:2 FTS) at 0, 80, 800, or 8000 ppb on a moss dry weight basis. Exposure to PFAS influenced final snout-vent length (SVL) and scaled mass index (SMI), a measure of relative body condition. Observed effects depended on species and chemical, but not concentration. Anurans exposed to PFOS, PFHxS (frogs only), and 6:2 FTS demonstrated reduced SVL versus controls, whereas salamanders exposed to 6:2 FTS showed increased SVL. Frogs exposed to PFHxS and 6:2 FTS and toads exposed to PFOS had increased SMI compared to controls; salamanders did not demonstrate effects. Concentrations of 6:2 FTS in substrate decreased substantially by 30 d, likely driven by microbial action. Perfluorooctane sulfonate had notable biota-sediment accumulation factors, but was still <1. Although a no-observable-effect concentration could not generally be determined, the lowest-observable-effect concentration was 50 to 120 ppb. Survival was not affected. The present study demonstrates that PFAS bioaccumulation from dermal exposures and sublethal effects are dependent on species, chemical, and focal trait. Environ Toxicol Chem 2021;40:717-726. © 2020 SETAC.