Abstract
Provision of feed containing arsenic may cause intestinal flora imbalance and consequently, the dysfunction of immunological protection of the cock. To understand the intricate tuning of immune responses in the intestinal tract elicited by subchronic arsenism, a cock model (1-day-old Hy-line cocks) was established by subjecting cocks to different environmentally relevant concentrations of arsenic in the diet for 90days. Intestinal cytokine and immunoglobulin (Ig) messenger RNA (mRNA) expression levels were determined using real-time PCR on days 30, 60, and 90. Results showed that, compared to those of the control groups, the mRNA levels of interleukin (IL)-1β, IL-2, IL-4, and interferon (IFN)-γ displayed increases on day 30 then decreases on days 60 and 90 dose-dependently in every tissue. Except for the decrease in the jejunum, the mRNA levels of IL-6 and IL-8 were increased in the duodenum, ileum, cecum, and rectum. However, the mRNA levels of IL-12β decreased in every tissue and every time point compared to those of the control groups. In contrast, chicks showed considerably higher expression levels of IgA, IgM, and IgG after exposure to arsenic. These results demonstrated that immune strategies of cocks were disturbed when suffered from subchronic arsenism, at least on the intestinal level.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
