Abstract
The DNA isolated from the tissues of the marine crab Cancer borealis can be separated into a major and a minor component (satellite DNA), the latter being primarily an alternating sequence of deoxyadenylate-deoxythymidylate (poly(dA-dT)). The location of the poly(dA-dT) was investigated by purifying subcellular fractions of hepatopancreas and testis, and by analyzing them for the presence of DNA by density gradient centrifugation and by thermal ultraviolet transition curves. Nuclei from hepatopancreas and from testis were shown to contain the same relative amount of poly(dA-dT) as whole hepatopancreas and whole testis. Hepatopancreas mitochondria were devoid of the poly(dA-dT), but contained an additional satellite DNA. These results suggest that the poly(dA-dT) of Cancer borealis is mainly of nuclear origin, and that the mitochondria contain an additional satellite DNA of intermediate density.
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