Subacute toxicity study of aqueous root extract of Terminalia schimperiana in male Wistar rats.

Abstract

The effect of administration of aqueous extract of Terminalia schimperiana root, "a medicinal plant", on some 'biomarker' enzymes, hematology parameters, liver function and kidney function parameters of rat cellular system was investigated. The aqueous extract was administered orally to male wistar rats (Rattus norvegicus) at various doses (1000, 2000, 3000 mg/kg body weight) daily for 21 days and the rats were sacrificed under chloroform anesthesia after 1, 7 and 21 days of oral administration. The administration of the aqueous extract of Terminalia schimperiana root for 21 days resulted in significant (P < 0.05) increase in packed cell volume and red blood cells level when compared with the control but were all within the normal test range. The differentials remained normal and the white blood cells level remained constant throughout the test period but increased after day 21 of the administration. Aspartate transaminase, alkaline phosphatase and acid phosphatase serum activities significantly (P < 0.05) increased, while the serum activities of alanine transaminase and gamma glutamyl transferase significantly (P < 0.05) reduced after 21 days of administration when compared with the control but they all fell within the normal test range. The extract produced (out of normal test range) significant (P < 0.05) increase in the serum albumin and total bilirubin. The kidney function parameters level was normal for sodium and potassium while the levels of creatinine and urea increased when compared with the control but were within the normal test ranges. The extracts did not have deleterious effect on the male wistar rat organs at the dosages investigated, therefore, studies for extended period is suggested to determine if the prolonged continuous use of the extract might cause challenge on the functional capacity of the organs.