Abstract
In vitro and in vivo methods of Raman spectroscopy have been developed to assess the degree of mineralization of the enamel of different functional groups. This article presents comparative studies that were carried out using scanning Raman microspectroscopy with various sources of laser excitation with wavelengths of 532, 785, and 1064 nm. It is shown that the intensity of Raman scattering of enamel can be a measure of its thickness. The obtained dependence of the Raman scattering intensity on the distance from the incisal edge is in good agreement with the literature data, where two independent methods (computer tomography and electron microscopy) are used to determine the enamel thickness values. The proposed methods can be considered as potential quantitative methods for express diagnostics of the state of tooth enamel in vivo.
Highlights
According to Global Disease Severity Survey 2016, at least 3.58 billion people suffered from oral diseases worldwide that year, and the most prevalent health disorder was dental caries in permanent teeth [1]
The results proved a possible use of this spectroscopy to determine accurately the enamel demineralization even at the early stages that are not visible to the eye
The results proved that the IR-Raman spectroscopy is informative to study the structural characteristics of the mineral components of dental hard tissues
Summary
According to Global Disease Severity Survey 2016, at least 3.58 billion people suffered from oral diseases worldwide that year, and the most prevalent health disorder was dental caries in permanent teeth [1]. The problem of diagnostics and prevention of caries development is socially significant. Various instrumental and hardware diagnostic methods are used to solve this problem. The methods of digital diagnostics applied in hardware research are more informative. It is necessary to consider the disadvantages of this technique: the negative effect of ionizing radiation, the impossibility to identify the initial stages of the carious process, and enamel demineralization [2]. There is another popular method that is the electric pulp test, which is used to determine the threshold of pulp sensitivity to electrical current.
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