Study the key biogenic amines in luncheon meat by capillary electrophoresis with indirect ultraviolet detection.

Abstract

In this work, a simple, accurate, and rapid capillary electrophoresis with an indirect ultraviolet detection method was developed to simultaneous separate 10 biogenic amines. It was found that β-cyclodextrin and ethylene diamine tetraacetic acid facilitated the separation of tryptamine and tyramine, spermidine and spermine, respectively. The optimized background electrolytes consisted of 20.0mmol/L imidazole, 8.0mmol/L β-cyclodextrin, 0.5mmol/L ethylene diamine tetraacetic acid, and 6.0% methanol (at pH 4.50 adjusted with acetic acid). The total analysis time of this method was less than 11min with limits of detection in the range of 0.14-1.98mg/L. The interday relative standard deviation of migration time and peak area were less than 1.36% and 4.44% (n=6), respectively. To verify the applicability, this method was carried out to analyze biogenic amines in commercial luncheon meat samples. Due to the complex composition of luncheon meat, the real samples were rinsed with deionized water to reduce the influence of matrices. It was found that both storage temperature and protein content of the luncheon meat samples affected the biogenic amines content during storage. The results of this study are instructive for the storage of high-protein meat products.