Study on the interaction between DNA and protein induced by anticancer drug carboplatin

Abstract

The interaction of DNA and human serum albumin (HSA) in the presence of anticancer drug carboplatin was studied with piezoelectric quartz crystal impedance (PQCI) and electrochemistry techniques. In the PQCI analysis, the correlative parameters including the frequency ( f 0), the motional resistance ( R m), and the static capacitance ( C 0) in the experiment were obtained and discussed in detail. Additionally, the kinetics parameters of the cross-linking process were investigated and a response kinetics model was deduced. The values of association rate constant k 1, dissociation rate constant k −1 and the reaction equilibrium constant K were estimated to be 1.895 (mg/ml) −1 s −1, 4.7 × 10 −5 s −1 and 4.03 × 10 4 (mg/ml) −1, respectively. Furthermore, cyclic voltammetry (CV) and electrochemical AC impedance techniques were employed to testify the cross-linking process.