Abstract
The fluorescence spectroscopic behavior of bromadiolone (anticoagulant rodenticide), a substituted 4-hydroxycoumarin derivative, was investigated in water and in organized media like micelles and cyclodextrins. A detailed study on various photophysical parameters like fluorescence intensity ( I F), quantum yield ( ϕ), lifetime ( τ) and steady state fluorescence anisotropy ( r) of bromadiolone in aqueous and in organized media was carried out. Bromadiolone in aqueous solution was observed to be in an aggregated state, thereby showing weak emission due to self-quenching. Marked enhancement of fluorescence intensity was observed in organized media like micelles and β-cyclodextrin. A preliminary investigation has been done to find out whether this enhancement of fluorescence can be used to develop a sensitive analytical method for determination of bromadialone in aqueous media. A linear relationship between the fluorescence intensity and concentration of bromadiolone was observed in the range of 0.15–7.9 μg ml −1 in cetyltrimethylammonium bromide (CTAB) and 0.5–26.4 μg ml −1 in β-cyclodextrin medium. The lower detection limit was found to be 37 ng ml −1 in presence of CTAB and 23 ng ml −1 in β-cyclodextrin. Comparison with 4-hydroxycoumarin, an unsubstituted analogue, was made.
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