Abstract
Tannery effluents having the different kind’s chemical compounds are extensively used to the production of leather industries and it’s considered as rich potential of environmental pollutant.Earthworms are easily affecting the toxic chemical in environmentally and in this organism is good experimental animal for monitoring the soil pollution and terrestrial ecosystem. In this study, we taken from the industrial raw tannery effluents and the experimental animal in earthworm species of Eudrilus eugeniae (10 for each group) were introduced to 48 hrs for tannery effluent in five different concentrations like 10 ml, 20ml, 30ml, 40 ml, and 50 ml and to find out LC50 level. The LC50 was found at 35 ml concentration. The study was used to the LC50/2, LC50, 2XLC50 for 48 hrs. Then the genotoxicity level was evaluated in Eudrilus eugeniae species. After that we collect earthworm’s coelomocytes by using the micronucleus (MN) test and comet assay (CA) test. Result of the study, MN and CA level was significantly increased in both genotoxicity and cytotoxicity assays and the high concentration of effluent to promote the increased level of DNA damage and micronucleus in Eudrilus eugenie species.
Highlights
Earthworms are chief decomposer of soil organic matter and aid in improving soil quality and fertility
Effects of tannery effluent on mean coelomocyte DNA damage score evaluated by the alkaline comet assay (CA) in coelomocytes of E. eugeniae are shown in Fig. 1 and Table 1
The LC50 for tannery effluent was found at 35 ml. significant difference (p< 0.05) was found between the mean DNA damage for LC50 and 2XLC50 as compared to the control group
Summary
Earthworms are chief decomposer of soil organic matter and aid in improving soil quality and fertility. About 3920 earthworm species are reported worldwide. Earthworms are widely used as model organisms in terrestrial ecosystem and they serve as a good indicator of heavy metal contamination due to their innate sensitivity to pollutants. Coelomocytes are immunocompetent cells which are affected by the toxic chemicals and are measured as a sensitive biomarker of environment health [2]. Tanneries are one of the most prominent sources of chromium pollution to the aquatic environment. A number of studies have been conducted to intricate the effects of these heavy metals on living organisms which provide damages by affecting the cell membranes, by altering the specificity of the enzymes, by the cellular functions and by damaging the structure of the DNA [9,10] (Chisti 2004; Ozer and Pirincci 2006)
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