Abstract
A method is described for growing pheochromocytoma in stationary culture. Pheochromocytoma cells were grown successfully for 22 days in Eagle's Basal Medium (EBME) and Morgan, Morton, and Parker's Medium No. 199 (M199) with retention of cellular metabolic characteristics. Evidence is presented confirming Blaschko's hypothesis on the biogenesis of adrenaline in man. Catecholamines as well as l-dopa (3,4-dihydroxyphenylalanine) were shown to be present in all tissue cultures assayed. These studies further indicate that, starting with l-tyrosine as precursor, the pheochromocytoma cells produce l-dopa, dopamine, noradrenaline, and adrenaline in tissue culture. A significant difference was found in the ratios of noradrenaline to adrenaline (NE/A) in the two media. The ratios of noradrenaline to adrenaline in EBME and M199 media were found to be 2.53 ± 1.46 and 0.60 ± 0.41, respectively. Further evaluation of these findings and the possible role of adenosine triphosphate (ATP) and l-methionine are included in this study.
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