Abstract
A high-performance capillary electrophoresis with amperometric detection (CE-AD) method has been developed for the analysis of seven bioactive ingredients, namely ferulic acid (FA), vanillin, vanillic acid, p-hydroxybenzoic acid, caffeic acid, gallic acid and protocatechuic acid, in Rhizoma Chuanxiong. The effects of several factors such as the acidity and concentration of running buffer, the separation voltage, the applied potential to working electrode and the injection time were investigated. Under the optimum conditions, the seven analytes could be well separated within 21 min at the separation voltage of 16 kV in a 60 mmol/L borax running buffer (pH 8.7). A 300 μm diameter carbon disk electrode has a good response at potential of +950 mV ( vs. SCE) for all analytes. Good linear relationship was established over three orders of magnitude with detection limits (S/N = 3) ranged from 3.3 × 10 −7 to 6.7 × 10 −9 g/mL. This proposed method has been successfully applied for the determination and comparison of different batches of Rhizoma Chuanxiong samples based on their characteristic electrochemical profiles.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
