Abstract
The nature and effects of bonded phases and analytical conditions on protein retention in high-performance hydrophobic-interaction chromatography (HIC) were investigated. Silica-based packing materials with different surface hydrophobicity were prepared and evaluated with respect to protein retention. The contact of proteins with the hydrophobic stationary phases caused conformational changes of proteins to some extent, but the extent of these changes was dependent on the hydrophobicity of the stationary phases, column temperature and the proteins themselves. Thermal behavior of some proteins in HIC on these phases is also shown.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
