Abstract
1. Carp with the dorsal aorta cannulated have been used to study the effects of a 5 hour exposure to methidathion at concentrations of 2 or 6 mg/litre. Blood samples taken during control, exposed and recovery periods were used to determine plasma acetylcholinesterase (AChE) activity, haematocrit value, mean cell volume, total plasma protein, and filtration time through Nucleopore filters containing pores of 8 microns. 2. A drastic inhibition of AChE activity was observed and this continued during the recovery for at least 5 days. Changes in other blood parameters were less marked and recovered soon after fish were returned to non-polluted water. The increased haematocrit and decrease in filtration time suggests some impairment of gas transfer during exposure but the depletion of AChE activity and associated muscular and neural disturbances are more serious results of pollution with this organophosphate insecticide.
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