Abstract
Suruhan Leaf (Paperomiapellucida L) is a weedy plant that is used by rural communities as herbal medicine. In this study, phytochemical tests have identified compounds contained in the ethyl acetate extract, then the toxicity test was carried out by the Brint Shrimp LethallytyTocycity (BSLT) method using Artemia Salina Leach shrimp larvae, an antibacterial activity test for bacteria: Bacillus cereus (ATCC) 11778), S. aureus (ATCC 25923), E. coli (ATCC 25922), S. saprophytic (ATCC 49907), Catsobacterfrendi (ATCC 18090), P.acne (ATCC 27853), Epidermis (ATCC 12228) with diffusion methods, various of concentration 1; 5; 10; 25% as control used chloramphenicol 30 mcg, the highest inhibitory zone at a concentration of 25% showed a strong antibacterial succession of 20.7; 11.1; 29.7; 15; 13.5; 10; 17.3 mm. The results of the identification of compounds in ethyl acetate extract are alkaloids, flavonoids, steroids, saponins, and tannins. The toxicity of LC50 is 71.28 ppm (toxic).
Highlights
Water leaves (PaperomiaPellucida L) are wild weed plants found in South America and Asia that are classified as a family of piperaceae, a place to grow around in damp shady places and enough water
Image of leaf Suruhan (Paperomia pellucida L) Secondary metabolites which identifiedof plants has been done by the phytochemical method [5,6,7,8]
Identification of the compounds obtained in the ethyl acetate extract of leaves paperomiapellucida L can be seen in the table 1
Summary
Water leaves (PaperomiaPellucida L) are wild weed plants found in South America and Asia that are classified as a family of piperaceae, a place to grow around in damp shady places and enough water. Erlenmeyers, measuring cups, beaker glass, stirring rods, watch glass, test tubes, pipettes, measuring flasks, tube racks, glass funnels, analytic balance of the sample due to direct contact with sunlight With such drying conditions, it is expected that the content of secondary metabolites contained in the sheets, hot plates, autoclaves, petri dishes, evendorf sample will not be damaged, after drying the leaves tube, vortex, paper disc, rotary evaporator, blender, are smoothed using a blender to expand the surface micropipette/stirring fan, incubator, autoclave, blender, fan, maceration equipment, maceration equipment, analytical balance, glassware, refrigerator, volume pipette. Materials The chemicals used in the study were obtained from chemical stores: hexane, chloroform, acetone, Test for the presence of flavonoid compounds was carried out by taking 0.5gram extracts, adding two drops of 5% FeCl3 solution to the drip plate ethyl acetate, ethanol Citric acid, MHA The color change to greenish or black and CM0337), MHB (HiMedia: GM 391), aquades, test blue indicates the presence of flavonoids
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