Study of enzymatic activity of microbiological biopreparation in the cultivation of cotton plant (Gossypium hirsutum L.) under saline stress conditions

Abstract

The changes in enzymic activity of the soils have been studied in the result of inoculation of cotton plants with microbiological preparation “Zamin-M” which was obtained on the base of association of strains Pseudomonas stutzeri SKB-308, Bacillus subtilis SKB-309 and Bacillus megaterium SKB-310 isolated from the rhizosphere of cotton plant grown in different saline soils. The results of the study showed that when the biopreparation “Zamin-M” was used, the enzymic activity of peroxidase was 1.76 ± 0.32 and in the control 1.5 ± 0.31 (mg purpurgalin/g soil, 24 hours), as well as the enzymic activity of polyphenol oxidase was 2.1 ± 0.53 and in the control 1.64 ± 0.51 (mg purpurgalin/g soil, 24 h). In the control variants, the activity of the enzyme of catalase relative to 1 g of absolutely dry soil was 1,0 ± 0,55 according to the amount of oxygen released in 5 minutes, while using the preparation its increase was observed and made 1.8±0.27 ml O2.Similar indicators were found in the study of invertase enzymic activity, when the preparation “Zamin-M” was applied, its amount constituted 271 ± 0.55 (mkg/absolutely dry soil), while in the control variant it made 111 ± 0.41 (mkg/absolutely dry soil). Urease enzymic activity was active under 24 ± 0.3 units when the preparation was used and in the control it was noted to be 14 ± 0,64 (mkg N/NH4/1 g soil) and phosphatase enzymic activity made 74±0.21 and in the control 53±0.32 (mkg P2O5/g soil, 24 hr) which led to the activation of biochemical processes in the soil.