Abstract
Calf thymus soluble deoxyribonucleoproteins (DNP) obtained by sonication of chromatins isolated both in a “physiological” salt medium and in a buffered water were resolved into four fractions on electrophoresis. The DNA extracted from DNP obtained in a salt medium occurs in fragments of discrete sizes while the sonicated chromatin gel obtained in a buffered water gives rise to a heterogeneous population of DNA fragments upon deproteinization. It is suggested that regularly spaced “weak” points exist in native chromatin and that the regularity is destroyed during isolation procedures involving the transfer of the nuclei into water.
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More From: Biochemical and Biophysical Research Communications
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