Abstract
目的 研究低浓度乙醇对肝癌细胞的影响及作用机制.方法采用噻唑蓝比色法观察乙醇对细胞增殖的影响;采用流式细胞仪法观察乙醇对肝癌细胞内活性氧的影响;采用琼脂凝胶电泳法观察DNA片断化条带.结果低浓度乙醇可明显抑制细胞增殖;乙醇作用于细胞后2 h内引起活性氧产生,24 h后细胞出现典型的凋亡表现.结论乙醇能够通过诱导细胞内活性氧的产生引起细胞凋亡,呈明显的剂量依赖性。
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