Studies on the relationship of vitamin D receptor gene distribution and bone mineral density in patients with maintenance peritoneal dialysis

Abstract

Objective To explore frequency distribution of vitamin D receptor (VDR) genotype and the correlations between polymorphism of VDR gene BsmⅠsites and bone mineral density in the patients undergoing maintenance peritoneal dialysis. Methods A case-control study was conducted based on 82 patients with maintenance peritoneal dialysis and 80 healthy people. The frequency distribution of VDR genotype in two groups was detected and analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Serum biochemistry and bone mineral density of the patients undergoing maintenance peritoneal dialysis were detected by dual energy X-ray absorption (DEXA) and automatic biochemical analyzer, respectively. All data was analyzed with SPSS 19.0 software. Results The frequency distribution of VDR genotype between case group and control group had no significant difference (P>0.05). The serum biochemical index such as Ca, P, alkaline phosphatase (ALP) and parathyroid hormone (PTH)] of patients with maintenance peritoneal dialysis had no significant difference among three VDR genotypes(P>0.05). Compared to VDR bb genotype, the serum Ca was significantly higher in VDR BB genotype (P<0.05). The bone mineral density (BMD) and T-Score of patients with VDR BB genotype were the lowest, the BB genotype was the highest, and the difference was statistically significant among three genotypes (P<0.05). Conclusions The frequency distribution of VDR genotype between case group and control group had no significant difference. However, there is a correlation between VDR gene BsmⅠsites and bone mineral density of maintenance peritoneal dialysis patients. Key words: Peritoneal dialysis; Receptors, calcitriol/GE; Bone density