Abstract
Abstract The effect of sodium hydroxide and sodium chloride alone as well as in combination on sludge protein solubilization was investigated. Sodium hydroxide treatment was found superior as a maximum protein solubilization (90%) was obtained by its use at pH 12.5. For the extraction of protein hydrochloric acid, sodium lignosulphonate, sulphuric acid, acetic acid and ammonium sulphate, as protein precipitating agents, were also tried. Of all the precipitants used ammonium sulphate (40%) was found most effective giving a maximum protein recovery (91%). Four parameters were mainly studied for the characterization of recovered protein. These include determination of total protein, moisture, ash and heavy metals. It was also found that protein recovery unit processes removed most of the metals originally present in the primary sludge and the recovered product contained all the essential amino acids which could serve as an animal feed supplement.
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