Abstract

1. Two high molecular substances, D-1 and D-2 described in this paper, were isolated from natural sake and identified chemically as some kinds of mucoprotein (N-containing polysaccharides).2. Preparing antiserum reacting with D-complex (D-1 and D-2), the amount of D-equivalent substance contained in commercial sake was determined serologically.3. The protein turbidity producing substances in sake were identified as D-complex; when the content exceeded 0.015% the turbidity phenomenon occurred.4. Most Aspergillus fungi produced D-complex even in synthetic media, but the fungi belonging to Penicillium, Mucor, and Rhizopus did not.5. The role of D-complex in sake taste was discussed.

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