Abstract
Commercial high methoxyl citrus pectin was purified by ultracentrifugation or ion exchange chromatography prior to GPC on Sepharose 2 B/Sepharose 4 B. The molecular weights by light scattering and the intrinsic viscosities of the fractions were determined. The effects of the purification procedures on the heterogeneity in shape are discussed by combining the Mark-Houwink plots with the universal calibration plot and the elution profile, respectively. Pectin fractions prepared by GPC are investigated in the same manner. GPC cannot differentiate between lower molecular weight extended coils and more compact high molecular weight molecules of increased polymer density. They are eluted together within a broad elution volume range.
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