Abstract
A simple colorimetric reaction for tryptophan has been adapted for use in determining the amount of γ-globulin in an antigen-antibody precipitate. The reaction is not interfered with by non-tryptophan-containing peptides or proteins. Values for the tryptophan content of unhydrolyzed rabbit and bovine γ-globulin and for unhydrolyzed bovine serum albumin emphasize the necessity for determining tryptophan content only on the intact protein and not on hydrolyzed material. The method has also been used to show that tyrosine-containing dipeptides do not bind non-specifically to γ-globulin to the extent of affecting the amount of γ-globulin determined by ultraviolet spectroscopy or Kjeldahl nitrogen.
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