Abstract
Purified kinetoplast DNA minicircles from Leishmania tarentolae promastigotes have been studied in terms of several physical properties: contour length and molecular weight, superhelix density of covalently closed molecules, buoyant density in CsCl, sedimentation coefficients, and thermal melting characteristics. The minicircles studied represented a class of free or loosely bound kinetoplast DNA molecules, but these were found to differ in no significant way from the minicircles isolated from sonicated kinetoplast DNA networks. Naturally occurring open minicircles were found to possess a single nick per molecule. Evidence was obtained for intramolecular heterogeneity in base composition as the cause of an observed multiphasic melting curve of open minicircles.
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