Abstract
Rat liver nuclei were fractionated into euchromatin and heterochromatin. Deoxyribonucleoproteins of these fractions were purified by continuous carrier-free electrophoresis and analyzed for their proteins, mainly the nonhistones. The ratio of nonhistones/DNA was found to be much higher in the purified deoxyribonucleoprotein of euchromatin than in the deoxyribonucleoprotein of heterochromatin. Comparative studies of the nonhistones derived from euchromatin and heterochromatin showed no clear indications for qualitative differences. By gel electrophoresis 13–14 bands were obtained, the molecular weight range was found to be in the range between 13 000 and 150 000. The main fractions of the nonhistones possessed isoelectric points from 4.5–6.5. The biological implications of these observations and the special methodical problems are discussed.
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