Abstract
The interaction between pyrimidine-annulated spiro-dihydrofuran (PSDF) with bovine serum albumin (BSA) was investigated following spectroscopic studies. The results indicate that dynamic quenching contributes to the fluorescence quenching of BSA by PSDF. The binding constant (K) and the number of binding sites (n) were calculated from the recorded spectra. Based on the Forster's non-radiative energy transfer theory, the average binding distance between BSA and PSDF was estimated. The synchronous fluorescence spectra and circular dichroism indicate that the conformation of BSA has been subjected to alteration in presence of PSDF. The thermodynamic parameters namely ΔH, ΔG, ΔS were calculated at different temperatures (20 o C, 30 o C, and 40 o C) and the results indicated van der Waals force and hydrogen bonding were predominantly present. The compound PSDF also found to exhibit antifungal activity against Aspergillus sp. In addition, the results obtained from molecular modeling calculation vividly elucidate the binding mode and the binding sites and results were not well in agreement with the experimental observations. Copyright © 2015 VBRI Press.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
