Abstract
The effect of hydrazine on the growth of Azotobacter vinelandii has been studied. It was found that small inocula fail to multiply in media containing hydrazine and that addition of hydrazine to proliferating cultures arrests growth. Both inhibitory effects are reversible. The rate of hydrazine disappearance from cultures of A. vinelandii depends directly on the density of the culture and is independent of initial concentration. The disapperance is due to the formation of a non-toxic, acid labile, hydrazine compound which accumulates in the medium surrounding the cells. Two coloured reaction products with picryl chloride, believed to be the picryl derivatives of this hydrazine compound, and a small amount of pyruvic acid were also isolated from such culture supernatants.
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