Abstract
The O-specific polysaccharide of Salmonella arizonae O45 ( Arizona 11) is acidic and has a branched hexasacharide repeating unit containing two residues of l-fucose, one residue each of d-galactose, d-ribose, d-glucuronic acid, and 2-acetamido-2-deoxy- d-glucose, and an O-acetyl group. It was studied with the help of 1H and 13C NMR spectroscopy, including 1D selective spin-decoupling and homonuclear Hartmann-Hahn spectroscopy, 2D homonuclear and 13C 1H heteronuclear shift-correlated (COSY) and NOE (ROESY) spectroscopy, as well as by methylation analysis, and selective cleavages with anhydrous HF (or dilute HCl) and lithium in ethylenediamine to yield two different tetrasaccharide fragments. As a result, the following structure of the polysaccharide was established: ▪ Anomalous 13C chemical shifts were observed in the spectrum of the trisaccharide fragment α- l-Fuc p-(1 → 2)-β- d-Gal p-(1 → 3)-β- d-Glc pNAc, structurally related to the Le d blood-group determinant, and rationalised by inter-residue proton-proton interactions.
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