Structure of 5S rDNA sequence of the gibel carp (Carassius gibelio) with 2n=100 chromosomes

Abstract

Event Abstract Back to Event Structure of 5S rDNA sequence of the gibel carp (Carassius gibelio) with 2n=100 chromosomes Aleksandra Szabelska1*, Lech Kirtiklis1 and Alicja Boron1 1 University of Warmia and Mazury in Olsztyn, Faculty of Biology and Biotechnology, Department of Zoology, Faculty of Biology and Biotechnology, Poland In vertebrate, the 5S ribosomal DNA (rDNA) consists of a conserved coding (transcriptional) unit of 120 bp (5S), which is separated from the next unit by a nontranscribed spacer (NTS). The coding sequence and the NTS together form a repeat unit which can be found in hundreds to thousands of copies tandemly repeated in the genomes. The coding region is highly conserved, even among nonrelated taxa, whereas the spacer region is variable, both in length and in sequence. Many studies have shown that birth-and-death processes can drive the evolution of 5S rDNA in distantly related taxa. The variations are due to insertions, internal subrepeats, deletions, base substitutions, pseudogenes, therefore the NTSs seem to be subject to rapid evolution. Thus, 5S rDNA sequences have been used as species – specific or population – specific genetic and cytogenetic markers in evolutionary studies: studying intraspecific variation, genome evolution, and phylogenetic reconstruction. Little is known about the nucleotide sequences and chromosomal location of 5S rDNA in teleosts in comparison with other vertebrate classes. Our preliminary research has related to diploid individuals of gibel carp (Carassius gibelio) with 2n=100 chromosomes. The PCR reaction has resulted in product 340 bp. Sequencing has yielded data on the exact nucleotide sequence and length of 5S rDNA fragment. Studies have shown no difference in length and nucleotide composition of the 5S sequence between males and females. Some study has revealed the influence of polyploidy on the organization and evolution of the 5S rDNA in teleosts fishes, therefore, then we will have studied the 5S rDNA sequences in triploid individuals. Acknowledgements The study was financed by a grant no. 12.610.006-300 of the UWM in Olsztyn Keywords: 5S rDNA, Carassius gibelio, Sequence Analysis, DNA, Sequence Alignment, Diploidy Conference: XV European Congress of Ichthyology, Porto, Portugal, 7 Sep - 11 Sep, 2015. Presentation Type: Poster Presentation Topic: Phylogeny, Systematics and Genetics Citation: Szabelska A, Kirtiklis L and Boron A (2015). Structure of 5S rDNA sequence of the gibel carp (Carassius gibelio) with 2n=100 chromosomes. Front. Mar. Sci. Conference Abstract: XV European Congress of Ichthyology. doi: 10.3389/conf.FMARS.2015.03.00187 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 30 Nov 2015; Published Online: 01 Dec 2015. * Correspondence: Ms. Aleksandra Szabelska, University of Warmia and Mazury in Olsztyn, Faculty of Biology and Biotechnology, Department of Zoology, Faculty of Biology and Biotechnology, Olsztyn, 10 – 718, Poland, aleksandra.szabelska@uwm.edu.pl Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Aleksandra Szabelska Lech Kirtiklis Alicja Boron Google Aleksandra Szabelska Lech Kirtiklis Alicja Boron Google Scholar Aleksandra Szabelska Lech Kirtiklis Alicja Boron PubMed Aleksandra Szabelska Lech Kirtiklis Alicja Boron Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.