Abstract
Three Sperm Activating Peptides (SAPs) have been isolated to homogeneity from the jelly coat of the eggs of a Japanese sea urchin, Anthocidaris crassispina , and two of them have been sequenced. At pH 6.8 they can stimulate the sperm respiration 20–30 fold, to the level in normal seawater (pH 8.2), and the half-maximal activation was achieved by SAPs as low as around 100 pM. The stimulative activity was both pH- and Na +-dependent. The chymotryptic fragments (res. 3–10) were 10 4–10 5 times less active, and the thermolytic fragment (res. 4–10) was 10 6 times less active than the parent SAP. CD spectra of SAPs indicate that they have unordered structure in aqueous solution.
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