Abstract
Abstract A cell wall polysaccharide preparation of Lactococcus lactis subsp. cremoris H414 was isolated by nitrous acid extraction, and purified by gel filtration and anion-exchange chromatography. Oligosaccharide fragments, obtained by partial acid hydrolysis or alkaline hydrolysis, were studied by means of monosaccharide analysis, methylation analysis, FAB-MS, EI-MS, and 1H NMR spectroscopy. In addition 1H, 13C, and 31P NMR spectroscopy, including two-dimensional homo- and heteronuclear measurements, were performed on the native polysaccharide. Combination of the various results demonstrated that in the polysaccharide preparation two repeating units with the structures [→2)-α-l-Rhap-(1→3)-α-d-Galp-(1→]n and [→6)-[α-d-Galp-(1→4)]-α-d-GalpNAc-(1→3)-β-d-GalpNAc-(1→1)-Ribitol-(4-phosphate→]m, respectively, can be recognized. So far, a covalent linkage between both polymers could not be confirmed nor be excluded.
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