Abstract
In this study, four malonyl isoflavonoid glycosides (MIGs), a type of isoflavonoid with poor structural stability, were efficiently isolated and purified from Astragali Radix by a medium pressure ODS C18 column chromatography. The structures of the four compounds were determined on the basis of NMR and literature analysis. Their major diagnostic fragment ions and fragmentation pathways were proposed in ESI/Q-TOF/MS positive mode. Using a target precursor ions scan, a total of 26 isoflavonoid compounds, including eleven malonyl isoflavonoid glycosides coupled with eight related isoflavonoid glycosides and seven aglycones were characterized from the methanolic extract of Astragali Radix. To clarify the relationship of MIGs and the ratio of transformation in Astragali Radix under different extraction conditions, two MIGs (calycosin-7-O-glycoside-6″-O-malonate and formononetin-7-O-glycoside-6″-O-malonate) coupled with related glycosides (calycosin-7-O-glycoside and formononetin-7-O-glycoside) and aglycones (calycosin and formononetin) were detected by a comprehensive HPLC-UV method. Results showed that MIGs could convert into related glycosides under elevated temperature conditions, which was further confirmed by the conversion experiment of MIGs reference compounds. Moreover, the total contents of MIGs and related glycosides displayed no obvious change during the long-duration extraction. These findings indicated that the quality of Astragali Radix could be evaluated efficiently and accurately by using the total content of MIGs and related glycosides as the quality index.
Highlights
Astragali Radix, named “huangqi” in Traditional Chinese Medicine, is the dried root of Astragalus membranaceus (Fisch.) Bge. or A. membranaceus var. mongholicus (Bge.) Hsiao [1]
With simple pretreatment procedures and a short separation time, a phytochemical investigation of Astragali Radix by medium pressure ODS C18 column chromatography (MPLC) led to the isolation of our malonyl isoflavonoid glycosides (MIGs) compounds
The results showed that Calycosin-7-O-Glc-600 -O-Mal (CYM) and Formononetin-7-O-Glc-600 -O-Mal (FMM) only transformed into related glycosides of CYG
Summary
Astragali Radix, named “huangqi” in Traditional Chinese Medicine, is the dried root of Astragalus membranaceus (Fisch.) Bge. or A. membranaceus var. mongholicus (Bge.) Hsiao (familyLeguminosae) [1]. Astragali Radix, named “huangqi” in Traditional Chinese Medicine, is the dried root of Astragalus membranaceus (Fisch.) Bge. or A. membranaceus var. As a commonly used natural herb, Astragali Radix has been listed in the Pharmacopoeia of many countries, including the Pharmacopoeia of the People’s Republic of China, the United States Pharmacopoeia, and Japanese Pharmacopoeia. It exhibits many biological functions, such as antioxidative [2,3], anticarcinogenic [4], immunomodulation [5], and anti-inflammatory activities [6], so it is frequently used to treat diabetes and related complications [7]. Isoflavonoids, as natural compounds that promote human health, were established to be important active components [10].
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
