Abstract
A water-soluble acidic polysaccharide containing 3-O-methyl galactose, named YB-PS4, was isolated from the twigs and leaves of Juniperus tibetica Kom. Its structure was characterized by monosaccharide composition analysis, methylation, and NMR spectroscopy. It was concluded that YB-PS4 had a backbone composed of→2,4)-α-Rhap-(1→, →3,5)-α-Araf-(1→, →2,4)-α-Galp-(1 → and →4)-α-GalpA-(1→, with branches of →2)-α-Rhap-(1→,→3)-α-Araf-(1 → and →2)-3-O-Me-α-Galp-(1→. The possible repetitive units were speculated and further analyzed by oligosaccharide analysis. YB-PS4 showed inhibitory effects on complement activation through the classical pathway (CH50 = 94.23 ± 8.90 μg/mL) and alternative pathway (AP50 = 194.76 ± 9.20 μg/mL). Preliminary mechanism study indicated that it interacted with C1q, C2, C3, C4 and C5. These studies pointed a way to understand the active constituents of J. tibetica and provided scientific bases for YB-PS4 as a complement inhibitor.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: International Journal of Biological Macromolecules
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
