Abstract
In this work, crude polysaccharides were extracted from longan fruit pericarp by hot water. After removal of proteins and purification by Sephadex G-100 gel filtration column, polysaccharides of longan fruit pericarp (PLFP) were subjected to structural identification. Gas chromatography analysis indicated PLFP comprised of L-arabinofuranose (32.8%), D-glucopyranose (17.6%), D-galactopyranose (33.7%) and D-galacturonic acid (15.9%). The glycosidic linkages were determined by methylation analysis and gas chromatography/mass spectrometry (GC/MS). The results showed that the backbone consisted of ?5)L-Araf-(1?, ?6)-D-Glcp-(1?, ?3)-D-Galp-(1?, ?3)-D-GalpA-(1? and ?6)-D-Galp-(1? with a molar proportion of 2:1:1:1:1. The infrared spectra and nuclear magnetic resonance (NMR) spectra further confirmed that the configuration of L-arabinofuranose was of a-form, while D-glucopyranose, D-galactopyranose and D-galacturonic acid were of b-form. The molecular weight of PLFP was measured to be 420 kDa by gel permeation chromatography. By determination of the anti-glycated activity, PLFP showed a good potential in inhibiting the glycation reaction in vitro.
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