Structural and techno-functional modifications of pea protein fractions by non-thermal technologies.

Pea protein globulins: Does their relative ratio matter?

Accelerating decarbonization in the food and beverage industry is critical to reducing greenhouse gas (GHG) emissions, as this sector accounts for approximately one-third of total emissions globally. Electrified, non-thermal food processing technologies offer promising alternatives to conventional thermal methods by improving energy efficiency and enabling cross-sectoral decarbonization. However, direct comparisons of their costs and environmental impacts remain limited due to the early-stage development of some technologies and variability in system configurations. This study provides a comprehensive review of four key non-thermal food processing technologies: high-pressure processing (HPP), pulsed electric fields (PEF), cold plasma, and ultraviolet light (UV). Using orange juice production as an illustrative case study, their industrial sustainability was evaluated through life cycle assessment (LCA) and technoeconomic analysis (TEA). Our LCA/TEA results show that, compared to conventional thermal pasteurization, orange juice processed with non-thermal technologies has slightly higher selling prices, with HPP being the most expensive. The carbon footprints of non-thermal processes are comparable to or lower than those of thermal pasteurization. This review offers valuable insights into the sustainability of various non-thermal food technologies, identifies key environmental and economic hotspots for industrial application, and serves as a guide for advancing sustainable practices in the food industry.

Fruits and vegetables have rich bioactive compounds and antioxidants that are vital for the human body and prevent the cell from disease-causing free radicals. Therefore, there is a growing demand for high-quality fruits and vegetables. Nevertheless, fruits and vegetables deteriorate due to their high moisture content, resulting in a 40–50% loss. Drying is a common food preservation technique in the food industry to increase fruits and vegetables’ shelf-life. However, drying causes chemical modifications, changes in microstructure, and bioactives, thus, lowering the final product’s quality as a considerable amount of bioactives compounds and antioxidants are lost. Conventional pretreatments such as hot water blanching, and osmotic pretreatment have improved fruit and vegetable drying performance. However, these conventional pretreatments affect fruits’ bioactive compounds retention and microstructure. Hence, emerging thermal (infrared blanching, microwave blanching, and high-humidity hot-air impingement blanching) and non-thermal pretreatments (cold plasma, ultrasound, pulsed electric field, and edible films and coatings) have been researched. So the question is; (1) what are the mechanisms behind emerging non-thermal and thermal technologies’ ability to improve fruits and vegetables’ microstructure, texture, and drying performance? (2) how do emerging thermal and non-thermal technologies affect fruits and vegetables’ bioactive compounds and antioxidant activity? and (3) what are preventing the large-scale commercialization of these emerging thermal and non-thermal technologies’ for fruits and vegetables, and what are the future recommendations? Hence, this article reviewed emerging thermal blanching and non-thermal pretreatment technologies, emphasizing their efficacy in improving dried fruits and vegetables’ bioactive compounds, structural properties, and drying performance. The fundamental mechanisms in emerging thermal and non-thermal blanching pretreatment methods on the fruits and vegetables’ microstructure and drying performance were delved in, as well as what are preventing the large-scale commercialization of these emerging thermal and non-thermal blanching for fruits and vegetables, and the future recommendations. Emerging pretreatment approaches not only improve the drying performance but further significantly improve the retention of bioactive compounds and antioxidants and enhance the microstructure of the dried fruits and vegetables.

Effects of nonthermal food processing technologies on food allergens: A review of recent research advances

Polysaccharides are natural polymer compounds widely distributed in plants, animals, and microorganisms, most of which have a broad spectrum of biological activities to promote human health. They could also be used as texture modifiers in food industry due to their excellent rheological and mechanical properties. Many researchers have shown that nonthermal processing technologies have numerous advantages, such as high extraction efficiency, short extraction time, and environmental friendliness, in the extraction of polysaccharides compared with the traditional extraction methods. Moreover, nonthermal technologies could effectively change the physicochemical properties and structural characteristics of polysaccharides to improve their biological activities or processing properties. Therefore, a comprehensive summary about the extraction and modification of polysaccharides by nonthermal technologies, including ultrasound, high hydrostatic pressure, pulsed electric fields, and cold plasma, was provided in this review. In particular, the underlying mechanisms, processing operations, and current application status of these technologies were discussed. In addition, the applications of combining nonthermal techniques with other technological methods in polysaccharide extraction and modification were briefly introduced.

Bioactive peptides (BPs) derived from animal and plant proteins are important food functional ingredients with many promising health-promoting properties. In the food industry, enzymatic hydrolysis is the most common technique employed for the liberation of BPs from proteins in which conventional heat treatment is used as pre-treatment to enhance hydrolytic action. In recent years, application of non-thermal food processing technologies such as ultrasound (US), high-pressure processing (HPP), and pulsed electric field (PEF) as pre-treatment methods has gained considerable research attention owing to the enhancement in yield and bioactivity of resulting peptides. This review provides an overview of bioactivities of peptides obtained from animal and plant proteins and an insight into the impact of US, HPP, and PEF as non-thermal treatment prior to enzymolysis on the generation of food-derived BPs and resulting bioactivities. US, HPP, and PEF were reported to improve antioxidant, angiotensin-converting enzyme (ACE)-inhibitory, antimicrobial, and antidiabetic properties of the food-derived BPs. The primary modes of action are due to conformational changes of food proteins caused by US, HPP, and PEF, improving the susceptibility of proteins to protease cleavage and subsequent proteolysis. However, the use of other non-thermal techniques such as cold plasma, radiofrequency electric field, dense phase carbon dioxide, and oscillating magnetic fields has not been examined in the generation of BPs from food proteins.

Comparison of the effects of high hydrostatic pressure processing and heat pasteurization on the techno-functional properties of Spirugrass®, a protein-rich microalgae extract

• Thermal and non-thermal technologies would eliminate pesticide residues. • Non-thermal technologies are superior to thermal technologies in pesticide residues decrease and quality attributes of fruits and vegetables retain. • Combined technologies are more effective than individual technologies. • Elimination efficiency is dependent on product types, pesticide characteristics, and operation parameters. • Green and pollution-free technologies and combined processing technologies are trends. Pesticide residues on food are threatening human health and wellbeing, ecological security. Food processing is one of the necessary ways to eliminate residues to guarantee the safety and sustainable development of the environment. This review outlines the mechanisms, applications, and factors influencing the efficiency as well as their limitations of pesticide residue elimination technologies. Conventional thermal processing technologies like drying, blanching, baking, and roasting have been proved to reduce pesticides extensively whereas sometimes concentration effects occur, and more toxic metabolites or by-products are generated. Additionally, the negative effects on quality attributes of fruits and vegetables (F&V) should be considered. Several innovative non-thermal processing technologies like ultrasound, cold plasma, high-pressure processing, and pulsed electric fields have flourished currently, which show great ability to eliminate pesticide residues significantly with minimal impact on the quality of F&V. In particular, heat-sensitive nutrients like ascorbic acid, phenolics, and carotenoids would retain to a great extent. Similarly, these technologies have their limitations. Furthermore, there is much information about combined processing technology affecting the pesticide behaviors of F&V. Finally, the future developments for pesticide elimination of these technologies are identified and discussed.

Stability of avocado paste carotenoids as affected by high hydrostatic pressure processing and storage

PurposeMillets are ancient grains, following wheat, that have been a fundamental source of human sustenance. These are nutrient-rich small-seeded grains that have gained prominence and admiration globally due to their super resilience in diverse climates and significant nutritional benefits. As millets are renowned for their nutritional richness, the demand for millet-based products increases. Hence, this paper aims in identifying the growing need for innovative processing techniques that not only preserve their nutritional content but also extend their shelf life.Design/methodology/approachIn traditional times, heat was the only means of cooking and processing of the foods, but the amount of damage they used to cause to the sensorial and nutritional properties was huge. Millets’ sensitivity toward heat poses a challenge, as their composition is susceptible to disruption during various heat treatments and manufacturing processes. To cater to this drawback while ensuring the prolonged shelf life and nutrient preservation, various innovative approaches such as cold plasma, infrared technology and high hydrostatic pressure (HPP) processing are being widely used. These new methodologies aim on inactivating the microorganisms that have been developed within the food, providing the unprocessed, raw and natural form of nutrients in food products.FindingsAmong these approaches, nonthermal technology has emerged as a key player that prioritizes brief treatment periods and avoids the use of high temperatures. Nonthermal techniques (cold plasma, infrared radiation, HPP processing, ultra-sonication and pulsed electric field) facilitate the conservation of millet’s nutritional integrity by minimizing the degradation of heat-sensitive nutrients like vitamins and antioxidants. Acknowledging the potential applications and processing efficiency of nonthermal techniques, the food industry has embarked on substantial investments in this technology. The present study provides an in-depth exploration of the array of nonthermal technologies used in the food industry and their effects on the physical and chemical composition of diverse millet varieties.Originality/valueNonthermal techniques, compared to conventional thermal methods, are environmentally sound processes that contribute to energy conservation. However, these conveniences are accompanied by challenges, and this review not only elucidates these challenges but also focuses on the future implications of nonthermal techniques.

This chapter presents some general aspects about high hydrostatic pressure (HHP), pulsed electric field (PEF), high voltage arc discharge (HVAD), and cold plasma (CP) and to explore the opportunities and drawbacks for the food and milk industry. HHP is an innovative technology for food preservation that protects the foods' sensory attributes and produces minimal quality loss. In addition, HHP has the potential to improve energy efficiency and sustainability of food production. PEF is a non-thermal technology that provides minimally processed, safe, nutritious and like-fresh foods to consumers. HVAD consists in application of electricity to pasteurize fluids by rapidly discharging electricity through an electrode gap, generating intense waves and electrolysis, thereby inactivating the microorganisms. CP is a novel non-thermal food processing technology that uses energetic, reactive gases to inactivate contaminating microbes on meats, poultry, fruits, and vegetables.

In the past decade, the plant-based meat alternative industry has grown rapidly due to consumers' demand for environmental-friendly, nutritious, sustainable and humane choices. Consumers are not only concerned about the positive relationship between food consumption and health, they are also keen on the environmental sustainability. With such increased consumers' demand for meat alternatives, there is an urgent need for identification and modification of protein sources to imitate the functionality, textural, organoleptic and nutritional characteristics of traditional meat products. However, the plant proteins are not readily digestible and require more functionalization and modification are required. Proteins has to be modified to achieve high quality attributes such as solubility, gelling, emulsifying and foaming properties to make them more palatable and digestible. The protein source from the plant source in order to achieve the claims which needs more high protein digestibility and amino acid bioavailability. In order to achieve these newer emerging non-thermal technologies which can operate under mild temperature conditions can reach a balance between feasibility and reduced environmental impact maintaining the nutritional attributes and functional attributes of the proteins. This review article has discussed the mechanism of protein modification and advancements in the application of non-thermal technologies such as high pressure processing and pulsed electric field and emerging oxidation technologies (ultrasound, cold plasma, and ozone) on the structural modification of plant-based meat alternatives to improve, the techno-functional properties and palatability for successful food product development applications.

Edible insects are an important source of proteins, fat, and chitin, which need to be extracted to develop tailored products with a controlled composition. Pulsed electric fields (PEF) is a non-thermal technology that can enhance the extraction. This study explores the effect of PEF on the extraction of protein, fat and chitin from cricket flour, as well as the material's functional properties. House crickets (Acheta domesticus) were treated with PEF at several conditions (4.9–49.1 kJ/kg). PEF treatment with 4.90 kJ/kg increased the extraction yields of protein (>18%) and fat >40%), while the treatment at 24.53 kJ/kg increased the oil binding and emulsifying capacity and antioxidant activity of the cricket flour by 28.10, 64.88 and 58.20%, respectively. Water binding capacity and foaming capacity were not affected by the PEF treatment. These results outline PEF as a suitable pretreatment for the valorization of house cricket biomass with possible industrial application.

Identification of equivalent processing conditions for pasteurization of strawberry juice by high pressure, ultrasound, and pulsed electric fields processing

ABSTRACTInterest in the development and adoption of nonthermal technologies is burgeoning within the food and bioprocess industry, the associated research community, and among the consumers. This is evident from not only the success of some innovative nonthermal technologies at industrial scale, but also from the increasing number of publications dealing with these topics, a growing demand for foods processed by nonthermal technologies and use of natural ingredients. A notable feature of the nonthermal technologies such as cold plasma, electrohydrodynamic processing, pulsed electric fields, and ultrasound is the involvement of external fields, either electric or sound. Therefore, it merits to study the fundamentals of these technologies and the associated phenomenon with a unified approach. In this review, we revisit the fundamental physical and chemical phenomena governing the selected technologies, highlight similarities, and contrasts, describe few successful applications, and finally, identify the gaps in research.