Abstract
IntroductionTamm-Horsfall glycoprotein (THP) and uromodulin are the most abundant glycoproteins in the urine of non-pregnant women and pregnant women, respectively. Today, the structural profile and biological activities of these glycoproteins are controversial. ObjectivesTo investigate the glycoforms and immunosuppressive activities of THP and Uromodulin MethodsUromodulin were isolated from diatomaceous earth filtration, N- and O-glycans were then analysed with mass spectrometer MALDI-TOF/TOF. MTS viability, cytokine and immunophenotyping assays were carried out to investigate the immunomodulatory activities of both THP and uromodulin Results & DiscussionMALDI-TOF/TOF analysis showed that both THP and uromodulin expressed high mannose and complex-type N-glycan carrying sialic acid residues, Sda, LacNAc and LacdiNAc sequence as the capping antennae. Uromodulin tends to express multiple degree of sialylation, in which up to four sialic acid residues were expressed on its tetra-antennary Nglycans. Core 1 and core 2 type O-glycans were observed in THP and uromodulin, with THP expressing more O-glycans. MTS viability assay showed that 125μg/ml of uromodulin and 250μg/ml of THP significantly reduced the PHA-activated PBMC cell viability, to 85.64 ± 5.9 > % and 84.59 ±2.94%, respectively. Uromodulin was 2-fold more active in suppressing PBMC cell viability. Immunophenotyping analysis showed that CD4+ T helper cells, CD8+ cytotoxic T cells, CD56+ NK cells and CD14+ monocytes were suppressed by THP. It induced pro-inflammatory cytokines such as IL-1β, TNF and Th1 cytokine IFN-γ. On the other hand, uromodulin had slightly greater effect in suppressing CD4+ T helper cells and CD8+ cytotoxic T cells. It only induced IL-1β and suppressed both Th1 cytokine IFN-γ and Th2 cytokine IL-10. Hence, uromodulin is more immunosuppressive than THP because it suppressed CD4+ and CD8+ T cells, stimulated IL-1β only and withheld the TNF secretion. ConclusionsGlycosylation changes as observed in THP are suggested to contribute to their differential immunosuppressive property. Developing
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
