Abstract
The seven rRNA operons (genes) of E. coli were physically mapped by restriction endonucleases, using the Southern blotting technique. Two complete rRNA operons (rrnB and rrnD) were isolated by the recombinant DNA method, using the pBR322 plasmid cloning vehicle. The rRNA operon carried by the transducing phage λdaroE152 was found to be a hybrid of the rrnD and rrnE operons. The DNA sequence of the promoter region for rrnB was determined. The RNA-polymerase binding sites on the cloned rRNA operons were localized by filter-binding and electron-microscopic techniques. rRNA transcription was investigated by electron microscopy and hybridization analysis of the in vitro transcripts. All these findings were correlated with the DNA sequence, and a tentative model was proposed to explain the unusual properties of rRNA promoters.
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