Abstract
Porphyran from the red seaweed Porphyra umbilicalis was degraded with β-porphyranase A derived from the marine flavobacterium Zobellia galactanivorans. β-Porphyranase A produces a new series of sulfated oligosaccharides that we characterized by NMR, HPAEC and size-exclusion chromatography. In contrast to previously used β-agarases, which produce predominantly non-sulfated oligosaccharides of the neoagarobiose series and hybrid oligosaccharides, β-porphyranase A produced oligosaccharides of the α- l-Gal p-6-sulfate (1 → 3) ß- d-Gal p (L6S-G) series and the L6S-G disaccharide was the major final product. The newly identified β-porphyranase A was further used in combination with β-agarase B to revise porphyran polysaccharide structure.
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