Abstract
Strong cation exchange chromatography: a new option for concurrent quantification of saxagliptin and metformin
Highlights
Several literatures have earlier reported the application of the most versatile C18 based chromatographic technique for the individual and concurrent quantification of saxagliptin (SXP) and metformin (MET) from the pharmaceutical product
Established chromatographic methods were evaluated in terms of their capabilities for concurrent estimation of both SXP and MET; included RP-HPLC (Gedawy et al, 2019), hydrophilic interaction liquid chromatography (HILIC) (AbuRuz et al, 2003; Peng et al, 2018), and capillary electrophoresis (CE) (Maher et al, 2019) techniques
The selected amines resolving capacity; resolution (Rs) and capacity factor (K’) was the optimum in strong cation exchange (SCX) method (60.52%), followed by RP-HPLC (50.79%) and CE while the lowest was observed for HILIC (50.83%); even it had the worst orthogonal selectivities relative to the RP-HPLC
Summary
Several literatures have earlier reported the application of the most versatile C18 based chromatographic technique for the individual and concurrent quantification of saxagliptin (SXP) and metformin (MET) from the pharmaceutical product. As realized, the utilization of this C18 is very challenging for several reasons Such as first, most negatively charged polar pharmaceutical amines do not retain in ion pairing mode (Gedawy, Al-salami & Dass, 2019; Gabhane et al, 2020) and, owing to their hydrophilic characteristics, it lowers their binding capacities to the ODS (Eva et al, 2016). Considering these aspects, strong cation exchange based liquid chromatography offers concurrent estimation of several amines to ensure and validate the comprehensive analytical studies. This strong cation exchange (SCX) based chromatography is complementary to the RP-HPLC which did not use the ion exchange mechanism. Multiple pharmaceutical drugs and natural products analysis usually required highly sophisticated methods, owing to the involvement with their complexities and most often, low abundance of UV-Visible detection sensitivity (Prasad et al, 2015; Yunoos and Sankar, 2015)
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