Abstract
Acceptance of capillary isoelectric focusing (cIEF) as a routine analytical method has been limited by variable migration times, irreproducible patterns, and sample precipitation. Poor separation reproducibility can be traced to a number of factors. In this paper we explore the possibility of eliminating reagents without buffering capacity, and the use of internal markers. An often overlooked optimization stage of cIEF is mobilization. We demonstrate that hydraulic mobilization can be finely tuned by manipulating the pressure applied, and that ion addition mobilization can be greatly improved by properly selecting the type of ion used and its concentration. By limiting the pH range of the ampholytes to 8.5 or less, hundreds of runs on an individual capillary are possible, which is a necessity for routine analysis.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
