Abstract
DNA damage in the A549 human lung cancer cell line treated with cold plasma irradiation was investigated. We confirmed that cold atmospheric plasma generated reactive oxygen and nitrogen species (RONS) in a liquid, and the intracellular RONS level was increased in plasma-irradiated cells. However, a notable decrease in cell viability was not observed 24 hours after plasma irradiation. Because RONS induce oxidative damage in cells, strand breaks and chemical modification of DNA in the cancer cells were investigated. We found that 8-oxoguanine (8-oxoG) formation as well as DNA strand breaks, which have been thoroughly investigated, were induced by plasma irradiation. In addition, up-regulation of 8-oxoG repair enzyme was observed after plasma irradiation.
Highlights
Cold atmospheric pressure plasma (CAP) has been intensively studied due to growing interest in biomedical applications
Several reactive oxygen and nitrogen species (RONS), e.g., OH radicals, cause DNA damage, which may be measured as strand breakage and/or chemical modification of DNA bases or 2-deoxyribose
We demonstrated DNA strand breaks and 8-oxoG generation that were induced by the He plasma jet
Summary
Cold atmospheric pressure plasma (CAP) has been intensively studied due to growing interest in biomedical applications. The biological influence of CAP treatment on living cells and organs needs to be well understood. Plasma-activated medium selectively kills glioblastoma brain tumor cells [7,8,9] and ovarian clear-cell carcinoma [10]. CAP treatment of cancer cells is expected to trigger a cancer-specific immune response [11, 12]. The common and central issues in this field are selective induction of apoptosis in cancer cells [13,14,15], the role of RONS generated during CAP treatment of cancer cells as the trigger of oxidative stress, and the different signaling pathways in cells [16,17,18,19,20].
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
