Abstract
A mince of wing tissue from pupae of the silkmoth, Hyalophora cecropia, in diapause was incubated in a physiological medium, and effects of hormones on biosynthesis were examined. Both RNA and protein synthesis, measured by incorporation of precursors for 1–2 hours, were significantly elevated after 20 hours with β-ecdysone. In stimulating RNA synthesis, β-ecdysone was effective at 1–20 μg/ml and less so at 0.2 μg/ml. At 5 μg/ml, β-ecdysone gave, on the average, 65% stimulation of RNA synthesis and was significantly more active than α-ecdysone (30% stimulation), while 22-isoecdysone had no effect.
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