Abstract
The sterols extracted from freeze-dried, log-phase cultures ofNeurospora crassa were separated by thin layer and analyzed by capillary gas liquid chromatography and mass spectrometry. Ergosterol, episterol, and ergosta-7,22 dienol were the major sterols of the free sterol fraction. The major esterified sterols, which constituted 4.95% of the total sterol fraction, were ergosterol, episterol, and ergosta- 7,22,24(28)-trienol, with lesser quantities of 4α-methyl-ergosta-8,24(28)-dienol. With the exception of lanosterol, all sterols were alkylated at the C-24 position.
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