Abstract
The steric course of the methyl group transfer to polygalacturonic acid to form the methyl ester group in pectin was studied using S-adenosylmethionine (AdoMet) carrying a methyl group made chiral by labeling with 1H, 2H, 3H, in an asymmetric arrangement. The incubation of the two diastereomers of this substrate with a particulate enzyme preparation obtained from Phaseolus aureus (mung bean) shoots gave the corresponding pectins. These were degraded in a series of stereochemically unambiguous reactions that converted the methoxy group into the methyl group of acetate, which was then analyzed for its configuration. The results indicate that the transfer of the methyl group from the sulfur of AdoMet to the oxygen of the carboxyl group proceeds with inversion of configuration of the methyl group.
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