Abstract
Shining club moss, Huperzia lucidula (Michaux) Trevisan, can be propagated in tissue culture, but it is difficult to obtain sterile explants. When standard disinfestation methods were used, 100% of H. lucidula apical shoot-tips disinfested for 10, 20, or 30 minutes with 1% sodium hypochlorite/0.15% Tween 20 became contaminated or browned within 3 weeks. Since this club moss is highly contaminated, a stepwise disinfestation procedure was developed to eradicate spore forming microbes. Based on the principle that vegetative microbial cells are more easily destroyed than spores, stepwise disinfestation induced spore germination before explant treatment with sodium hypochlorite. A 48-hour disinfestation treatment resulted in 50% explant survival without visible contamination. Fewer explants (22%) were successfully disinfested when a 24-hour stepwise disinfestation was used. Applying the stepwise disinfestation method to contaminated cultures after a standard disinfestation protocol produced an additional 15.6% contamination-free club moss shoot-tips. After successful surface disinfestation, H. lucidula shoot-tips and gemmae established in vitro grew normally.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
