Abstract
Root explants of Eustoma grandiflorum were bombarded with plasmid pBI221 harboring the uidA gene encoding β-glucuronidase (GUS) driven by a cauliflower mosaic virus 35S promoter, and the effects of pre- and post-bombardment culture in various starvation media on the number of blue spots of GUS-expressing cells were studied. It was found that pre- and post-bombardment culture in nitrogen-depleted media increased the expression frequency of the transgene, by 4- to 8-times that of the control, and that 3-h pre-culture in the nitrogen-depleted medium gave the highest expression frequency.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
