Abstract
The present study deals with standardization of an in-house standard preparation and three marketed samples of Brahmi vati, which is a traditional medicine known to be effective in mental disorders, convulsions, weak memory, high fever and hysteria. Preparation and standardization have been done by following modern scientific quality control procedures for raw material and the finished products. The scanning electron microscopic (SEM) analysis showed the reduction of metals and minerals (particle size range 2-5 µm) which indicates the proper preparation of bhasmas, the important ingredient of Brahmi vati. Findings of EDX analysis of all samples of Brahmi vati suggested the absence of Gold, an important constituent of Brahmi vati in two marketed samples. All the samples of Brahmi vati were subjected to quantitative estimation of Bacoside A (marker compound) by HPTLC technique. Extraction of the samples was done in methanol and the chromatograms were developed in Butanol: Glacial acetic acid: water (4.5:0.5:5 v/v) and detected at 225nm. The regression analysis of calibration plots of Bacoside A exhibited linear relationship in the concentration range of 50-300 ng, while the % recovery was found to be 96.06% w/w, thus proving the accuracy and precision of the analysis. The Bacoside A content in the in-house preparation was found to be higher than that of the commercial samples. The proposed HPTLC method was found to be rapid, simple and accurate for quantitative estimation of Bacoside A in different formulations. The results of this study could be used as a model data in the standardization of Brahmi vati.
Highlights
Ayurveda, the ancient system of plant based medicines is gaining recognition throughout the world and many herbal drugs are clinically tested and accepted for manufacturing (Gold frank L, et al 1982; Vulto A.G, et al 1988)
These four samples were subjected to physico-chemical, pharmaceutical and energy dispersive X ray spectroscopy analysis (EDX)
scanning electron microscopic (SEM) analysis of bhasma and pisties showed the reduction in particle size of minerals and metals in the range of 25μm (Figure 2)
Summary
The ancient system of plant based medicines is gaining recognition throughout the world and many herbal drugs are clinically tested and accepted for manufacturing (Gold frank L, et al 1982; Vulto A.G, et al 1988). Ayurvedic medicines prepared in the form of tablets or pills are known as Vati and Gutika. These are made of one or more drugs of plant, animal or mineral origin. One batch of BV was prepared in laboratory with standard materials (IBV) and three commercial samples (BV1, BV2 and BV2) from market were collected. The amount of Bacoside A — one of the saponine of Brahmi — was analyzed quantitatively in all four samples These parameters can be compared with any commercial formulation available in the market for evaluating its purity and quality. The scientists assumed bacoside A to be an indicator of the presence of brahmi (Marker compounds of selected Ayurvedic drugs, 2009)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: African Journal of Traditional, Complementary and Alternative Medicines
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
