Abstract
A continuous system, employing the Davis (5) chamber buffer, was found to give superior resolution of the hemoglobin system of Chironomus tentans when compared with the same author's dise gel system. This improvement in resolution was found with at least one other continuous buffer system. The accuracy of the system employed was found to be 0.015 R f unit around the mean value for a given protein peak. This system can be adapted to micro electrophoresis with a 13.0× reduction in the amount of Hb required for direct scanning of unstained gels at 412 nm, reducing the Hb requirement to 2.5 μg per gel run. This direct scanning system was applicable to nucleic acids. Micro electrophoresis was, however, only 1.6 times as sensitive as the standard type in this region, requiring approximately 1.0 μg per run in comparison to the 1.6 μg required for the standard type.
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