Abstract
Oligodendrocytes generate distinct patterns of myelination throughout the CNS. Variations in myelination along axons may enable neurons to fine-tune conduction velocities and alter signal synchronisation. Here we outline a staining protocol permitting the assessment of the number and length of myelin sheaths formed by oligodendrocyte in the mouse grey matter. This protocol enables the investigation of myelination without the need for reporter mice or technically challenging protocols, aiding the investigation of factors influencing myelin production in the brain.
Highlights
[Background] Oligodendrocytes generate the insulating myelin sheaths around axons in the central nervous system (CNS)
Oligodendrocytes are located throughout the CNS, not all axons are myelinated suggesting that the formation and size of myelin sheaths may precisely permit the fine-tuning of action potential speeds and neuronal synchronisation (Pajevic et al, 2014)
Understanding what influences the amount of myelin generated by oligodendrocytes is important for understanding how myelin alters neuronal function
Summary
[Background] Oligodendrocytes generate the insulating myelin sheaths around axons in the central nervous system (CNS). Understanding what influences the amount of myelin generated by oligodendrocytes (i.e., the number and size of sheaths formed by one cell) is important for understanding how myelin alters neuronal function. This allows the complex individual morphologies of oligodendrocytes to be traced in the sparsely myelinated grey matter of the mouse cortex enabling the assessment of myelin sheath number and length. Low gelling temperature agarose (Sigma-Aldrich, catalog number: A9414) 15.
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